+49 (089) 18 93 668 0 - Info@ScienceServices.de   Wir sind für Sie erreichbar von: Mo. - Do. 09:00-17:00, Fr. 09:00-15:00
Sprache

Keyence VHX Fluorescence Images

(0)
Leider können wir keine passenden Produkte zu ihrer Auswahl finden.

Humane Dermale Fibroblasten (adhärente Zellen) auf Collagen-Scaffold

  • Zellen wurden zunächst 2 Tage auf Collagen-Scaffold wachsen gelassen in Multi-well-Platte
  • Anschließend Anfärbung der Zellen mit Calcein-Acetoxymethylester für 15 min (Fluoreszenzfarbstoff, färbt vitale Zellen, Konzentration 1 pM, Anregungswellenlänge 494 nm / Emmissionwellenlänge 517 nm)
  • Entfernen des Färbemediums und Waschschritt, dann Zugabe von reinem Medium
  • Scaffolds verbleiben in Multi-well-Platte, Mikroskopie durch das Medium (ca. 5 mm)

Mikroskop: Keyence VHX 900F, Z100 Objektiv, 100x Vergrößerung, ausgestattet mit NightSea Fluoreszenz-Kit mit Filtersatz Royal Blue

100x
400x

 

Electronic component failure analysis

Examining electronic components that were embedded in epoxy, cross-sectioned, polished, and highlighted with a fluorescent epoxy-like dye. Images were made with the Keyence VH-ZST lens. While the manufacturer of the fluorescent dye recommended excitation with ultraviolet light, our Royal Blue light head was used for these images, and was found to be superior to ultraviolet. You can read more about this kind of inspection application in the article on this web site.

30x
200x
1000x
2000x

 

Circuit board conformal coating

Conformal coating on a circuit board, with damage. Images made with Keyence VH-ZST lens. The upper row was made with Ultraviolet light, the ‘traditional’ wavelength range for this application. While this works well with the excitation directed from the side, the ultraviolet wavelengths do not work well with the ZST lens when the light head is positioned over the fiber optic input port. The lower row shows the same areas under excitation with our Violet light head. While the fluorescence is marginally less bright at lower magnifications, this source works better through the Keyence lens optics, producing the brighter area you can see in the 200x image.

Ultraviolet excitation
30x
50x
100x
 200x
Violet excitation
30x
50x
100x
200x

 

Concrete thin section

The images below were made from a 20μm concrete thin section with the Keyence VH-Z100 lens, NIGHTSEA Royal Blue excitation. As with the electronic components above, the manufacturer of the fluorescent dye recommended excitation with ultraviolet light. Our Royal Blue light head was used for these images and was found to be superior to ultraviolet. You can read more about this kind of inspection application in the article on this web site.

100x
200x
500x
700x
Thin section courtesy of Department of Mineralogy and Geochemistry, Institute of Geoscience and Geography, University Halle-Wittenberg, Germany.

 

Epoxy on a motor shaft

Small motor shaft with epoxy where it does not belong. Imaged with Keyence VH-ZST lens, 20x magnification.

Epoxy on motor shaft, 20x
 
 
 

 

Gel defect in a nylon granule

Nylon 6,6 granules can manifest a process defect called ‘gel’. If there is too much gel in a production batch it can compromise downstream production. You can read more about this in our application article.


Images made with Keyence VH-ZST lens, Royal Blue excitation. At 100x and above the images utilized the Keyence automated focus stacking capability.

50x
200x
300x
700x

 

Fluorescent-dosed plastic particles

Fluorescent-dosed plastic particles, 38 – 45 microns. Image made with ultraviolet excitation. Image courtesy of an explosives detection company.

 

Naturally fluorescent mineral

This mineral sample, collected at the Sterling Hill Mine in Ogdensburg, New Jersey, contains willemite (green fluorescence), calcite (red fluorescence) and franklinite (black – no fluorescence).

Willemite, 20x
Willemite/calcite boundary,
composite 20x and 500x
Willemite/calcite boundary, 20x Willemite/calcite boundary, 50x
 
Willemite/calcite boundary, 500x
Willemite, composite 20x and 1000x
Fluorescent mineral –
Willemite, 2000x
Willemite, 1000x

 

Ammonite fossil

Sectioned and polished fossil of an ammonite, an early (and now extinct) cephalopod, related to modern-day nautilus, squid, cuttlefish, and octopus.

White light
Fluorescence
Ammonite fossil fluorescing under the Keyence microscope, VH-ZST lens, Royal Blue excitation.
20x
100x
200x
500x

 

Wax verification

Verifying proper wax (torque modifier) application on parts from a subcontractor under UV excitation.

Wax verification under UV excitation
Wax verification under UV excitation