Cryo Sectioning and the Use of Diamond Knives
- Electron Microscopy of vitrified ultrathin sections allows the cell struture to be studied in the hydrated state.
Richter, Karsten., Gnagi, Helmut., Dubochet, Jacques. (1991). A Model for Cryosectioning Based on the Morphology of Vitrified Ultrathin Sections. J. of Microscopy 163, 19-28.
- It is demonstrated that cryosectioning with a diamond knife in conjunction with an ionization electrode can produce good cryosections by optimizing the cutting parameters (i.e. sectioning temperature, mechanical stability of the sample, and sectioning velocity).
Reference: Michel, M., Gnagi, H., Muller, M. (1992). Diamonds are a Cryosectioner’s Best Friend. J. of Microscopy 166, 43-56.