+49 (089) 18 93 668 0 - Info@ScienceServices.de   Wir sind für Sie da! Rufen Sie uns an oder fordern Sie   hier einen Rückruf an.
Sprache

EMbed-812 Kit

Ersatz für das nicht mehr erhältliche Epon-812. Ergibt die gleichen Resultate und besitzt die gleichen physikalischen Eigenschaften.
Artikel für gruppiertes Produkt
Artikelname Menge
EMbed-812 Epoxid Einbett-Kit mit BDMA

E14121

174,80 € 208,01 €
EMbed-812 Epoxid Einbett-Kit mit DMP-30

E14120

178,80 € 212,77 €
EMbed 812, Harz, Einzelkomponente, 450ml

E14900

86,50 € 102,94 €
EMbed 812, Harz, Einzelkomponente, 4x450ml

E14901

322,00 € 383,18 €
DMP-30, 2,4,6-Tri(Dimethylaminomethyl)-Phenol, 25ml

E13600

15,20 € 18,09 €
NMA, Methyl-5-Norbornen-2,3-Dicarbonsäureanhydrid, 450ml

E19000

57,30 € 68,19 €
BDMA, Benzyldimethylamin, 100ml

E11400

52,60 € 62,59 €
D.D.S.A., Dodecenylbernsteinsäureanhydrid, Speziell destilliert, 450 m

E13710

84,20 € 100,20 €

Produktdetails

Beschreibung

EMbed 812 Resin for Microscopy EMbed 812 is our replacement for EPON 812, the most widely used embedding resin for electron mi-croscopy, which was discontinued in 1978. EMbed 812 provides the same excellent preservation and cutting qualities as EPON 812, and may be substituted in all similar formulations.

Luft (1961) established EPON 812 as a reliable embedding medium, excellent both for plant and animal tissue. The same advantages offered by EPON 812, are offered by EMbed 812: rapid penetra-tion, greater contrast, easy sectioning, stability under the electron beam, satisfactory staining of most thick sections for light microscopy and thin sections for electron microscopy.

 

Recommended Procedure

Fixation: Tissues can be fixed in a wide range of fixatives. One of the more commonly used fixatives is an alde-hyde (i.e.: glutaraldehyde) followed by osmium tetroxide.

 

Dehydration: There are many different dehydration schedules that can be followed. A typical one is as follows:

70% Ethanol for 10 minutes
100% Ethanol for 10 minutes
100% Ethanol for 15 minutes
100% Propylene Oxide for 15 minutes
100% Propylene Oxide for 15 minutes

Note: Longer times may be required for some samples.

Mixing Instructions:

  Small Amount Medium Amount Large Amount
Mixture A:      
EMbed 812

5ml

20ml 62ml
DDSA 8ml 31ml 100ml
Mixture B:      
EMbed 812 8ml 20ml 100ml
NMA 7ml 17ml 90ml

 

Final Embedding Mixture:

  Small Amount Medium Amount Large Amount
Mixture A 13ml 51ml 162ml
Mixture B 15ml 37m 190ml
DMP-30* 0.42-0.56ml 1.3-1.7ml 5.3-7.0ml

*For better penetration and stability BDMA is recommended in place of the DMP-30. The quantity of BDMA which is required is 2.5-3% while DMP-30 is 1.5-2%.

It is much simpler when mixing EMbed 812 to use a one-step single mix formula. The following formu-lations may be used depending on the desired hardness of the block:

  Soft Medium Hard
EMbed 812 20ml 20ml 20ml
DDSA 22ml 16ml 9ml
NMA 5 ml 8 ml 12 ml
DMP-30 .70-.94ml 
or 1.18-1.4ml (BDMA)
.66-.88ml
or 1.1-1.3ml (BDMA)
.62-.82ml
or 1.0-1.2ml (BDMA)

 

Slight variations of the accelerator (DMP-30 or BDMA) will drastically affect the color and brittleness of the block.

Prior to measuring and mixing the resin and the anhydride should be warmed (60°C) to reduce their viscosity. Immediately before use, the two mixtures (A&B) are blended, and the accelerator added in the above mentioned proportion. Thorough mixing is imperative to be able to achieve uniform blocks.

While preparing EMbed 812, the hardness of the block can be varied to suit various sectioning condi-tions depending on the ratio of mixture A and mixture B in the final embedding mixture. An increase in the proportion of mixture B will make the block harder. A mixture of 1:1 has proven most successful for general use. All components of the kit should be kept at room temperature in tight stoppered bottles.

Although the mixture can be stored for up to 6 months at 4°C it is highly recommended that freshly prepared embedding medium always be used. If you choose to store the mixture you should warm it thoroughly prior to adding the accelerator.

Infiltration: It is recommended that for all of the infiltration steps a specimen rotator be used.

  1. Drain the tissue of most of the propylene oxide, leaving a little so the tissue does not dry out.
  2. Replace the solvent with a 1:1 solution of propylene oxide:embedding medium and allow it to stand for at least 1 hour at room temperature.
  3. A second change of 2:1 embedding medium to propylene oxide at room temperature overnight is recommended.
  4. Remove the mixture, replace it with 100% embedding medium and leave for 30 minutes-2 hours at room temperature.

 

Embedding: This may be done in EMS embedding capsules (EMS Catalog #70020) or a flat embedding mold (EMS Catalog #70900).

Transfer each sample to a dry capsule or mold and fill the mold with embedding medium. Cure the medium in an oven at 60°C for 24 hours.

Blocks can be trimmed and sectioned after the blocks return to room temperature.

Reference: Luft, J.H.(1961), J. Biophys. Biochem. Cytol. 9, 409.

Weitere Informationen

Weitere Informationen
Anwendung
Einbettung
Basis
Epoxy
Gebotszeichen
Augenschutz benutzen Augenschutz benutzen
Handschutz benutzen Handschutz benutzen
Signalwort
Gefahr
Symbol GHS
GHS02 GHS02
GHS05 GHS05
GHS07 GHS07
GHS08 GHS08
GHS09 GHS09
Gefahrenhinweise
H226
H302
H302_H312_H332
H314
H315
H317
H319
H334
H335
H411
H412
Sicherheitshinweise
P273
P280
P310
P321
P330
P362
P301_312
P303_361_353
P304_340
P305_351_338
P332_313
P333_313
P405
P501
Bestandteile 450ml EMbed-812, 450ml DDSA, 450ml NMA, 50ml DMP-30 / BDMA
Verpackungseinheit kit
Versandklasse
Gefahrgut LQ
Lagertemperatur
RT
Arbeitsbereich
Elektronenmikroskopie
Hersteller
EMS
SDB (mehrsprachig) 14900, 13710
SDB (mehrsprachig) 19000, 13600
SDB (mehrsprachig) 11400

Downloads