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EMS GloQube® Plus & Zubehör - Glimmentladungs-System für TEM Grids

Der EMS GloQube® Plus ist ein neu konzipiertes Glimmentladungs-System mit zwei unabhängig voneinander arbeitenden Vakuum-Kammern. Entwickelt zum Hydrophilisieren von Oberflächen ist der GloQube® Plus ideal für die Behandlung von TEM Grids, beschichteten TEM Grids oder anderen Oberflächen geeignet, die hydrophobe Eigenschaften aufweisen. Vakuumpumpe nicht enthalten.
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Produktdetails

Beschreibung

Rapid, reliable results with the NEW EMS GloQube® Plus

Unique Dual Chamber Processing, Safe Handling of Reagents

The GloQube® Plus has two independent vacuum chambers:

  1. Clean chamber, designed for applications requiring hydrophobic/hydrophilic conversion, typically using air as the process gas
  2. Vapor chamber, designed for use with reagents such as methanol and alkylamine. With operator safety firmly in mind, reusable septum-sealed reagent vials are used. Loading and removing reagents is convenient and reliable – the vial, located in its holder, is inserted into a shielded needle using a simple bayonet fitting.

To prevent accidental damage, the high voltage lead is shielded. The plasma current is variable by adjustment of the vacuum level using an argon leak valve with the plasma voltage being preset. For maximum sputter coating efficiency, the gas injector system ensures that argon gas enters the chamber close to the plasma discharge. Venting is to argon.

 

 

Key Features

  • Automatic control of vapor and air introduction
  • Flush and purge cycles of vapor chamber and gas line
  • Two chambers for separate in-air and in-vapor processes without contamination*
  • Negative and positive discharge modes
  • Safe vapor delivery using septum-sealed vials
  • Automatic valving between chambers to prevent cross-contamination
  • Fast and/or soft venting options
  • Single door for easy sample loading
  • Adjustable three height sample stage
  • Intuitive touch screen control
  • Loaded with typical standard recipes
  • Password protected user profiles and programmable user recipes

The primary application of the EMS GloQube® is the hydrophilization (wetting) of carbon-coated TEM support films and grids which otherwise have the tendency to be hydrophobic. Glow discharge treatment with air will make film surfaces negatively charged and hydrophilic and allow the easy spread of aqueous solutions. This and other processes are outlined below.

Glow Discharge Process

Surface State Charge Atmosphere Typical applications
Hydrophilic Negative Air Hydrophilization and cleaning of carbon coated TEM grids
Hydrophilic Positive Air* – with magnesium acetate post-treatment Nucleic acid adhesion to carbon films
Hydrophobic** Positive Alkylamine Controlled orientation and improved adhesion of
negatively charged proteins, antibodies and nucleic acids
Hydrophobic** Negative Methanol Controlled orientation and improved adhesion of
positively charged protein molecules
(e.g. ferritin, cytochrome c)

* Air followed by post-treament with magnesium acetate by the user.
** Hydrophobic, as noted above, may represent a hydrophilic sample of less than 90 degree contact angle.

Unique Dual Chamber Processing, Safe Handling of Reagents

The GloQube® has two independent vacuum chambers:

  1. Clean chamber, designed for applications requiring hydrophobic/hydrophilic conversion, typically using air as the process gas
  2. Vapor chamber, designed for use with reagents such as methanol and alkylamine. With operator safety firmly in mind, reusable septum-sealed reagent vials are used. Loading and removing reagents is convenient and reliable – the vial, located in its holder, is inserted into a shielded needle using a simple bayonet fitting.

To prevent accidental damage, the high voltage lead is shielded. The plasma current is variable by adjustment of the vacuum level using an argon leak valve with the plasma voltage being preset. For maximum sputter coating efficiency, the gas injector system ensures that argon gas enters the chamber close to the plasma discharge. Venting is to argon.

 

Easy sample loading, fast turnaround times

Each chamber can accommodate two 25 x 75 mm glass microscopes slides. Loading could not be easier using draw-style chamber doors and specimen stages. The stages are height adjustable and fitted with removable glass slide holders. For additional convenience – and to allow easy access for chamber cleaning – the stages can be completely removed.


Touch screen control – rapid data input, simple operation

The intuitive touch screen allows multiple users to rapidly input and store preferred process "recipes". Typical default glow discharge protocols are loaded as standard. Additionally, help files and useful maintenance data such as system on time and time since last clean are readily available to the operator. An Ethernet communications port is included for software updates.


Vacuum, automatic valving and controlled venting

The GloQube® has automatic valving between chambers which maintains cleanliness by preventing cross-contamination. At the end of a process run, automatic soft venting to atmosphere through filtered inlets ensures TEM grids are not disturbed. The GloQube® requires a single vacuum pump working in the 0.1 to 1 mbar range. A typical pump time to operational vacuum is 60 seconds.

 

Accessory kit included consisting of the following:

750 mm long flexible stainless steel vacuum tube with clamps, fuses, glass vials, vial caps and sealing washers, needle (spare).


Specifications

Power and Processes

Plasma current 1-50 mA
HV power supply 30W
Maximum voltage 800V
Electrode polarity – clean chamber DC glow positive DC glow negative
Electrode polarity – vapor chamber DC glow positive DC glow negative
Sample stage 125 x 100 mm with location for two glass slides 25 x 75 mm or a 36 place grid holder
Sample stage operational heights Adjustable 12.5 mm (0.5"), 22.5 mm (0.9") or 35 mm (1.38")
Pump hold time requirement 0-72 hours
Process time 1-900 seconds


Safety

Chamber vent inlets Filtered air inlets with slow vent to minimise sample disturbance
Onboard reagent storage Reagents (e.g. methanol or alkylamine) are contained in reusable sealed glass vials to minimize exposure to hazards.
High voltage safety interlocks Hardware safety interlocked and software for process control


Vacuum

Vacuum control Intergrated pirani gauge
Working vacuum range 0.1 to 1 mbar
Vacuum pump minimum requirements 5 m3/hr, 3600 l/m, 0.03 mbar ultimate vacuum. Inlet flange: KF 16
Pumping time Typical pump time to an operational vacuum of 0.1 mbar in 60 seconds
Vacuum isolation Isolation valves to switch vacuum and prevent process chamber cross-contamination


User Interface

User interface Full graphical interface with touch screen buttons and controls. In addition to displaying profiles, parameters, help screen and maintenance information are available
Profiles and profile logging Capability to store 100 user profiles (name, date, time, vacuum, current and polarity)


Dimensions and Communications

Chamber size 100 mm W x 100 mm H x 127 mm D (3.94" x 3.94" x 5")
Instrument size 336 mm H x 364 mm D (13.2" x 14.3")
Instrument weight 19.5 kg (42.9 lbs)
Pump (optional) 391 mm W x 127 mm D x 177 mm H (15.4" x 5" x 7")
Pump weight 16 kg (35.3 lbs)
Footprint with optional pump 366 mm W x 600 mm D x 336 mm H (14.4" x 23.6" x 13.2")
Power requirements 120 V 60 Hz, 15 A or 230 V 50 Hz, 10 A
Instrument power rating 100-240 V AC 60/50 Hz 700 VA including pump, IEC inlet
Optional pump power rating 115/230 V 60/50 Hz 450 W
Communication port USB interface

Weitere Informationen

Weitere Informationen
Anwendung
EM
REM
TEM

Wird oft gemeinsam gekauft