Tech Notes
Tech Notes für EM und LM Anwendungen (auch bekannt als “Kirsch Notes” von Electron Microscopy Sciences)
Sie möchten gerne wissen, wie man Formvar-befilmte Grids selbst herstellt oder warum Acetonitril eine gute Alternative für Propylenoxid ist?
Die Antworten auf diese und viele andere Fragen, sowie viele andere hilfreiche technische Tipps und
Produktinformationen für Anwendungen aus den Bereichen Lichtmikroskopie und Elektronenmikroskopie finden Sie hier bei den “Tech Notes”.
(Mit freundlicher Genehmigung von Stacie Kirsch, EMS)
General Lab and Safety Tips
General Tips and Protocols for Sample Preparation for Microscopy (LM/EM)
Light Microscopy and LM Specimen Handling
Tissue Staining for Histology (LM)
Buffering, Fixation and Dehydration of Tissues (LM/EM)
Preparation of Karnovsky's Fixative (PDF)
Providing Solutions: Buffers for Electron Microscopy (PDF)
Providing Solutions: Dehydrants for Electron Microscopy (PDF)
Providing Solutions: Fixatives for Electron Microscopy (PDF)
Use of Paraformaldehyde, Glutaraldehyde and Acrolein Buffer
Fixative Solution for Fixing Cornea
Prevent Paraformaldehyde Static Jumps
Surgical Gloves - A Safety Tip -for usage with Glutaraldehyde
Staining of Tissues with Heavy Metals (EM)
Post-Staining of Sections with Heavy Metals (EM)
Resin Embedding of Samples (EM)
Providing Solutions: TEM Embedding Resins (PDF)
Providing Solutions: Epoxy and Acrylic Resins (PDF)
Embedding Samples in Epoxy and Acrylic Resins (PDF)
Preparation of Embedding Mixtures
Epoxy Resins: Problems, Causes, and Solutions
Embed812 - an Epon-812 Replacement
Low Viscosity Kit Formation with ERL 4221
Use of Accelerators for Epoxy Resins
The Use of Flexibilizers and Plasticizers
The Use of DMP-30 (2,2-Dimethoxy Propane)
Quetol and the Addition of Water
Embedding Resin Stain Permability
Storage of Embedding Resin Mixtures
Reference: Re-Embedding of Tissue Poorly Embedded
Reference: Re-Embedding of Paraffin-Embedded Specimen Tissue in Resin
Adherence of LR White Sections to Glass Slides for Silver Enhancement Immugold Labeling
LR White Technical Tips - Distinguishing LR White from Tissue
LR White Technical Tips - Adjusting pH
LR White Technical Tips - Improved Polymerization of Acrylic Resins
Ultramicrotomy and Sectioning Tips (LM/EM)
TEM Grid and Section Handling Tips (EM)
Grids: Shiny Side vs Dull Side?
Grids: Removing a Charge from the Surface of Grids
Reference: Simple Method for Handling Grids
Techniques for Retrieving Thin Sections (PDF)
Section Pick-up with the Perfect Loop
Improving the Adhesion of Sections Filmless Grids
Removal Of Lead Citrate & Uranyl Acetate Precipitates on LR White Sections
Mounting of SEM Samples (EM)
Critical Point Drying (EM)
Cryofixation of Tissues (EM)
Immunolabeling (LM/EM)
The Use of Polyethylene Glycol
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