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Quetol 651-NSA

Eine wasserlösliches Einbettmedium mit geringer Viskosität und guten Infiltrationseigenschaften auf Epoxydharzbasis für Licht-und Elöektronenmikroskopie.

Ab 11,20 € 13,33 €

Artikel für gruppiertes Produkt
Artikelname Menge
Quetol 651 - NSA Epoxid Einbett-Kit

E14640

410,70 € 488,73 €
DMP-30, 2,4,6-Tri(Dimethylaminomethyl)-Phenol, 25ml

E13600

11,20 € 13,33 €
NMA, Methyl-5-Norbornen-2,3-Dicarbonsäureanhydrid, 450ml

E19000

47,00 € 55,93 €
NSA, Nonenylbernsteinsäureanhydrid, destillierte EM-Qualität, 450ml

E19050

62,70 € 74,61 €
Quetol 651 Harz, 225ml

E20440

277,00 € 329,63 €

Produktdetails

Beschreibung

Quetol 651 is a water miscible epoxy resin with a low viscosity affording easy embedding, infiltration, sectioning and staining. Characterized by low electron scattering, it can be used for both light and electron microscopy.

Semi-thin sections approimately 0.2um thick embedding in QUETOL 651 are suitable for examination with an ordinary electron microscope operating at 100 kV.

This method uses QUETOL 651 (EMS Catalog #20440) and the epoxy resin, Nonenyl Succinic Anhy-dride (NSA) (EMS Catalog #19050) and NMA (EMS Catalog 19000) as hardener and DMP-30 (EMS Catalog #13600) as accelerator. QUETOL 651 is an ethylene glycol diglycidyl ether, an epoxy resin of low viscosity. It is a light-colored epoxy resin with a viscosity of 15 cps at 25 degrees C. It is readily miscible with water, alcohol, acetone, n-butyl glycidyl ether, etc. It acts as a dehydrating agent, and combines chemically in any cured epoxy resin formulation.

The following mixture is recommended for this method:

QUETOL 651 35 ml
NSA 54 ml
NMA 11ml
DMP-30 1.5-2.0 ml

This mixture has a low viscosity, and is therefore easy to handle and penetrates readily and complete-ly into the specimen. It sets in about 24 hours at 60 degrees C and yields a light yellow block which is suitable for sectioning at 20 degrees C. This final hardness of the blocks can be adjusted by altering the ratio of QUETOL 651 and NSA.

Tissue sample sizes are approximately 2 x 2 x 1 mm, since a section of an area of approximately 2 x 2 mm is suitable for mounting on a grid 3 mm in diameter. The pieces are fixed in buffered aldehyde so-lution, postfixed in buffered osmium tetroxide solution, washed in a non-phosphate buffer, and should then be stained en block with aqueous uranyl acetate. After dehydration in graded alcohol or QUETOL 651, the following infiltration procedure is appropriate. n-Butyl glycidyl ether (n-BGE) is employed as an auxiliary to infiltration. All steps are carried out on a shaker at room temperature.

Embedding:

Dehydrating agent /n-BGE (1:1) 30 minn
BGE 30 minn
BGE/QUETOL 651 mixture (1:1) 1-2 hr.
QUETOL 651 mixture 2-3 hr.

Embed in gelatin (or polyethylene) capsules and cure in an oven at 60 degrees C for about 24 hours.

Reference: Kushida, H. and Kushida, T. Journal of Electron Microscopy, Vol 31, No. 2, 206-209, 1982.

Weitere Informationen

Weitere Informationen
Anwendung
LM
TEM
Basis
Epoxy
Gebotszeichen
Augenschutz benutzen Augenschutz benutzen
Handschutz benutzen Handschutz benutzen
Signalwort
Achtung
Symbol GHS
GHS07 GHS07
GHS08 GHS08
Bestandteile 225ml Quetol 651, 450ml NMA , 450ml NSA, 50ml DMP-30
Verpackungseinheit kit
Eigenschaft
wasserlöslich
Versandklasse
Gefahrgut LQ
Lagertemperatur
0-5°C
SDB (mehrsprachig) E20440, E19000
SDB (mehrsprachig) E19050
SDB (mehrsprachig) E13600

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